Role of motility in adherence to and invasion of a fish cell line by Vibrio anguillarum.
نویسندگان
چکیده
To understand further the role of the flagellum of Vibrio anguillarum in virulence, invasive and adhesive properties of isogenic motility mutants were analyzed by using a chinook salmon embryo cell line. Adhesion was unaffected but invasion of the cell line was significantly decreased in nonmotile or partially motile mutants, and the chemotactic mutant was hyperinvasive. These results suggest that active motility aids invasion by V. anguillarum, both in vivo and in vitro.
منابع مشابه
The ability of marine Bacillus spp. isolated from fish gastrointestinal tract and culture pond sediment to inhibit growth of aquatic pathogenic bacteria
In this research, antagonistic activity of two Bacillus species isolated from digestive tract of marine fish and culture pond of sea cucumber was studied. The inhibitory activity of Bacillus spp. isolates against some common pathogenic bacteria of fish was assessed using the agar diffusion method. The strain of B. subtilis G024 exhibited antimicrobial activity against Vibrio anguillarum, V. har...
متن کاملThe ability of marine Bacillus spp. isolated from fish gastrointestinal tract and culture pond sediment to inhibit growth of aquatic pathogenic bacteria
In this research, antagonistic activity of two Bacillus species isolated from digestive tract of marine fish and culture pond of sea cucumber was studied. The inhibitory activity of Bacillus spp. isolates against some common pathogenic bacteria of fish was assessed using the agar diffusion method. The strain of B. subtilis G024 exhibited antimicrobial activity against Vibrio anguillarum, V. har...
متن کاملInfluences of temperature, salinity and starvation on the motility and chemotactic response of Vibrio anguillarum.
The role of growth factors for the motility and chemotaxis of the fish pathogen Vibrio anguillarum was determined. Cells of V. anguillarum were chemotactic to serine in the temperature range 5-25 degrees C and in 0.8-2.7 % NaCl. The chemotactic response was significantly higher at 25 degrees C than at 5 or 15 degrees C. Growth in medium with 1.5 % NaCl gave a higher response than growth with 3 ...
متن کاملRpoN of the fish pathogen Vibrio (Listonella) anguillarum is essential for flagellum production and virulence by the water-borne but not intraperitoneal route of inoculation.
To investigate the involvement of RpoN in flagellum production and pathogenicity of Vibrio (Listonella) anguillarum, the rpoN gene was cloned and sequenced. The deduced product of the rpoN gene displayed strong homology to the alternative sigma 54 factor (RpoN) of numerous species of bacteria. In addition, partial sequencing of rpoN-linked ORFs revealed a marked resemblance to similarly located...
متن کاملThe Effects of NDRG2 Overexpression on Cell Proliferation and Invasiveness of SW48 Colorectal Cancer Cell Line
Background: Colorectal cancer (CRC) is one of the most common causes of cancer-related death in the world. The expression of N-myc downstream-regulated gene 2 (NDRG2) is down-regulated in CRC. The aim of this study was to investigate the effect of NDRG2 overexpression on cell proliferation and invasive potential of SW48 cells.Methods: SW48 cells were transfected with a plasmid overexpressing ND...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of bacteriology
دوره 182 8 شماره
صفحات -
تاریخ انتشار 2000